![]() McClelland M, Nelson M, Raschke E (1994) Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nelson M, Raschke E, McClelland M (1993) Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases. Plant Cell Rep 27:617–631īoyko A, Kathiria P, Zemp FJ, Yao Y, Pogribny I, Kovalchuk I (2007) Transgenerational changes in the genome stability and methylation in pathogen-infected plants: (virus-induced plant genome instability). Clin Chim Acta 363:165–176Īgarwal M, Shrivastava N, Padh H (2008) Advances in molecular marker techniques and their applications in plant sciences. Wu YY, Csako G (2006) Rapid and/or high-throughput genotyping for human red blood cell, platelet and leukocyte antigens, and forensic applications. Nagamura Y, Antonio BA, Sasaki T (1997) Rice molecular genetic map using RFLPs and its applications. Kochert G (1991) Restriction fragment length polymorphism in plants and its implications. Gusella JF, Wexler NS, Conneally PM, Naylor SL, Anderson MA, Tanzi RE et al (1983) A polymorphic DNA marker genetically linked to Huntington’s disease. Todd R, Donoff RB, Kim Y, Wong DT (2001) From the chromosome to DNA: Restriction fragment length polymorphism analysis and its clinical application. Indian J Biochem Biophys 26:285–288īarnes SR (1991) RFLP analysis of complex traits in crop plants. Pethe V, Lagu M, Chitnis PK, Gupta V, Ranjekar PK (1989) Restriction fragment length polymorphism: a recent approach in plant breeding. Restriction fragment length polymorphism (RFLP).Here, we describe RFLP analysis using methylation-insensitive and methylation-sensitive enzymes. Since DNA can be digested with methylation sensitive enzymes, the assay can also be used to analyze a methylation pattern of a particular locus. The assay involves restriction enzyme digestion of DNA followed by hybridization of digested DNA using a radioactively or enzymatically labeled probe. The comparison of RFLPs allows investigators to analyze the frequency of occurrence of mutations, such as point mutations, deletions, insertions, and gross chromosomal rearrangements, in the progeny of stressed plants. RFLPs are typically detected as DNA fragments of different lengths after digestion with various restriction endonucleases. Restriction fragment length polymorphism (RFLP) is a difference in DNA sequences of organisms belonging to the same species.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |